293 Transfection

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Mon Jun 8 04:45:15 EST 1998

Kevin, using PBS w/ Ca2+ could improve results, too. It might enhance 
integrin interaction with extracellular matrix, my supervisor 
recently noted.


> I am transfecting the 293 cells with Lipofectamine Plus.  In my
> analysis, whether staining or harvesting for quantitation, it get a
> lot of cells lifting.  I have found that warmed PBS does alleviate
> some of the cell loss, but I was wondering if anyone has tried using
> a cocktail of matrix proteins, or any other technique with better
> success
> Kevin
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Wolfgang Schechinger         
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm

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