RNA and protein purification from same sample

Ahmet Hoke hoke at ACS.UCALGARY.CA
Mon Jun 8 12:05:15 EST 1998


Hi,
Recently I've started to play around with RT-PCR and I'm using TriZol
method to purify my RNA. Has anyone also tried to obtain the protein
from the white buffy coat during the purification step? If so, how is
the yield and are there any protocols for it?
Thanks
--
Ahmet Hoke MD, PhD, FRCPC
University of Calgary
Department of Clinical Neurosciences




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