Blunt ending??

jalali at my-dejanews.com jalali at my-dejanews.com
Mon Jun 8 11:39:51 EST 1998


I need to blunt end a linearized plasmid with a 5'
overhang of 2 bases, as well as a fragment of DNA with a 5' overhang.
The strategy I'm considering at the moment is digestion with Mung-bean
nuclease. Any thoughts?

Also, assuming this strategy is okay, will I need to kinase, so that I can
ligate afterwards.

Any info would be greatly appreciated.

Farid Jalali
Mushroom Research Group
University of Toronto at Mississauga

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