test: do not read

Bjørn Olav Kvamme Bjorn.Kvamme at mbi.uib.no
Mon Jun 8 09:10:42 EST 1998


Test from lab 1

Lab.1 wrote:

> I tried to detect a plasmid in mouse blood with PCR (primer for
> amp-region). I treated the blood samples with Microwave, as Ohhara et al
> described in BioTechniques 17:726-728 (1994), but the blood inhibited
> the PCR-reaction. I did not use the same PCR-mix or programme, but my
> plasmid and no blood containing pos. control is nice.....if I add
> plasmid to the blood, it doesn`t work anymore....
>
> Please, help me.
>
> Christoph Hotz-Behofsits
>
> Exchange student at
>
> University of Bergen / Department of Molecular Biology / Norway






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