PCR of Blood after Microwave-treating

[Lab.1]

I tried to detect a plasmid in mouse blood with PCR (primer for
amp-region). I treated the blood samples with Microwave, as Ohhara et al
described in BioTechniques 17:726-728 (1994), but the blood inhibited
the PCR-reaction. I did not use the same PCR-mix or programme, but my
plasmid and no blood containing pos. control is nice.....if I add
plasmid to the blood, it doesn`t work anymore....

Please, help me.

Christoph Hotz-Behofsits

Exchange student at

University of Bergen / Department of Molecular Biology / Norway