PCR of Blood after Microwave-treating
Christoph.Hotz-Behofsits at mbi.uib.no
Mon Jun 8 10:56:49 EST 1998
I tried to detect a plasmid in mouse blood with PCR (primer for
amp-region). I treated the blood samples with Microwave, as Ohhara et al
described in BioTechniques 17:726-728 (1994), but the blood inhibited
the PCR-reaction. I did not use the same PCR-mix or programme, but my
plasmid and no blood containing pos. control is nice.....if I add
plasmid to the blood, it doesn`t work anymore....
Please, help me.
Exchange student at
University of Bergen / Department of Molecular Biology / Norway
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