PCR of Blood after Microwave-treating

Lab.1 Christoph.Hotz-Behofsits at mbi.uib.no
Mon Jun 8 10:56:49 EST 1998


I tried to detect a plasmid in mouse blood with PCR (primer for
amp-region). I treated the blood samples with Microwave, as Ohhara et al
described in BioTechniques 17:726-728 (1994), but the blood inhibited
the PCR-reaction. I did not use the same PCR-mix or programme, but my
plasmid and no blood containing pos. control is nice.....if I add
plasmid to the blood, it doesn`t work anymore....

Please, help me.

Christoph Hotz-Behofsits

Exchange student at

University of Bergen / Department of Molecular Biology / Norway




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