RNA preps - looking for suggestions
theo.thijs at med.kuleuven.ac.be
Mon Jun 8 08:06:22 EST 1998
If you don't care about the RNA yield why don't you try TriZol buffer?
Matthew L. Beyers wrote in message <35794891.BC6CC3D7 at achilles.net>...
I'm currently doing GITC/Phenol/Chloroform RNA preps using Corex #8445 30 ml tubes and doing my spins at 10,000 RPM in a Sorvall SA-600 rotor.
I'm having a bit of a problem though in that sometimes the tubes shatter during the spin, meaning that I lose all of my sample. I've called Fisher and they've said that the people that make Corex (Corning?) have discontinued that tube and that there is no replacement available.
I was hoping that someone out there would have an idea for a substitute for these tubes that is reasonably easy to see through (like the glass of Corex) able to withstand phenol and chloroform and the high (~16,000 RCF) g forces necessary. Any ideas?
Thanks a bunch,
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