RNA preps - looking for suggestions

[Matthew Beyers]

Theo Thijs wrote:
> 
> If you don't care about the RNA yield why don't you try TriZol buffer?
> 

Well, actually, I do care about the yield and I've tried Trizol and such
in the past.  Recalling that a perfect RNA sample should have an
A260/A280 ratio of about 2.0, I've done no better than achieve 1.4 with
Trizol.  However, the prep I'm using ALWAYS gives me 2.0 +/- 0.02.

So my conclusion is that, to save two extra pipetting steps, people are
paying huge amounts of money for the convenience of Trizol.  Personally,
I think it's a shame to waste valuable research money in this way. 
However, that's another matter.  My only problem is that my tubes
shatter.  Several people have suggested I go to Nalgene Oakridge tubes
and I think I'll give this a whirl.

Ta ta,

Matt