RNA preps - looking for suggestions
mbeyers at science.uottawa.ca
Mon Jun 8 20:15:23 EST 1998
Theo Thijs wrote:
> If you don't care about the RNA yield why don't you try TriZol buffer?
Well, actually, I do care about the yield and I've tried Trizol and such
in the past. Recalling that a perfect RNA sample should have an
A260/A280 ratio of about 2.0, I've done no better than achieve 1.4 with
Trizol. However, the prep I'm using ALWAYS gives me 2.0 +/- 0.02.
So my conclusion is that, to save two extra pipetting steps, people are
paying huge amounts of money for the convenience of Trizol. Personally,
I think it's a shame to waste valuable research money in this way.
However, that's another matter. My only problem is that my tubes
shatter. Several people have suggested I go to Nalgene Oakridge tubes
and I think I'll give this a whirl.
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