Extracting lagre plasmid (~80kb) from Pseudomonas strain

io30634 at maine.maine.edu io30634 at maine.maine.edu
Tue Jun 9 08:01:15 EST 1998


Hi,
Does any have a working method (besides CsCl) for extracting large
plasmid DNA from Pseudomonas strain? If you do, please let me know
where I can find it. I tried alkaline lysis and the boiling method
both are not working. Thanks in advance.
Waiki



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