Secreted proteins

J.S Good jsg at badger.bio.cam.ac.uk
Tue Jun 9 10:01:28 EST 1998


Hi

I want to screen the genome of a gram-negative pathogen for secreted
proteins, perhaps by cloning genomic DNA fragments into an E. coli
expression plasmid, placing the fragments upstream of a reporter gene.
Have you had any experience with this, or can you suggest a suitable
promoter/reporter/plasmid?

Any advice welcome.

Cheers
James



P.S. For email, substitute mole for badger.



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