lambda gt22 sequencing primers...
siyer at bs.jhmi.edu
Mon Jun 8 10:58:23 EST 1998
i need to sequence my inserts that is in a lambda gt22 vector. i have
searched genbank to see if the vector is online, but i couldnt find it.
i figure i can just cut it out using the unique sites in the
multicloning site , but i dont know how the library was made i.e which
enzymes were used. i can probably just use the first most and last most
restriction sites as primers, but i think my yield in a PCR reaction is
gonna be piss poor if i do it that way. does anyone have any idea as to
how i can get a sequence map of this phage vector? the only phage
vector that is available online is the original lambda phage, but it has
been thru a billion mutations and manipulations to generate this gt22
vector (which is a gt11 variant with extra sites). lambda gt11 is also
not online, as far as i have checked. also, anyone have any knowledge
of any companies that might sell premade gt22 sequencing primers??
thanks much and please reply to siyer at bs.jhmi.edu .
Sai Iyer (siyer at bs.jhmi.edu)
Pre-doctoral fellow i.e lowly graduate student
Dept. of Biological Chemistry
Johns Hopkins University School of Medicine
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