co-immuno-precipitation

xianghuai lu xianghuai.lu at ccc.uab.edu
Wed Jun 10 13:29:35 EST 1998


Hi, groups:

  I am doing an experiments trying to co-IP two proteins and then do
western blot to check them. One protein is 57 KD (CD5) and the other is
100 KD (Ap2). It looks like I can use larger one to bring down smaller
one but not the other way around. The other way is what we wanted. Does
anyone have an better idea to do IP or any tricks?

Any suggestion will be greatly appreciated.

Lu




More information about the Methods mailing list