Vent Pol. for blunting

Cres Bookstein cbookste at MEDICINE.BSD.UCHICAGO.EDU
Wed Jun 10 13:27:40 EST 1998

Vent, despite claims to contrary, leaves a proportion of A overhangs (you
can use it with TA cloning vectors).  It is not as efficient for blunting
as PFU.  Otherwise, it is a great proofreading enzyme.

Crescence Bookstein, Ph.D.
Research Associate/Assistant Professor
Dept of Medicine-GI  mc 6084
Univ. of Chicago
5841 S. Maryland Ave.
Chicago, IL 60637
cbookste at

Phone: 773/702-2283             Fax: 773/702-2281

More information about the Methods mailing list