Expression of peptides in E.Coli

Iolaus at my-dejanews.com Iolaus at my-dejanews.com
Wed Jun 10 08:28:26 EST 1998


I would be interested as well

Kevin




In article <6llj85$2us at bioalp.biobase.dk>,
  kjaer at biobase.dk (Svend Kjaer) wrote:
>
> Hi all,
>
> I am in need of a peptide of the length of roughly 20 amino acids. Instead of
> having someone to synthesize it (who I will then have to pay), I thought of
> expressing it into the periplasme of E.coli and purifying it via a His-tag.
> However, small non-structured peptides of that length may be degraded by pro
> teases, so I would like to know if anyone has experience with such set-ups
>
> Thanks,
>
> Svend Kjaer,
>
> IMSB,
> University of Aarhus,
> Denmark
>


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