Expression of peptides in E.Coli

Alejandro Krimer a.krimer at biosidus.com.ar
Wed Jun 10 08:20:11 EST 1998


Dear Sven:

	I think it will be cheaper (If you make the count about your
work time),
	to pay the synthesis
	Good luck!!!

							Alejandro
	 ----------
> From: 	kjaer at biobase.dk[SMTP:kjaer at biobase.dk]
> Sent: 	Wednesday, June 10, 1998 6:22AM
> To: 	methods at net.bio.net
> Subject: 	Expression of peptides in E.Coli
> 
> Hi all,
> 
> I am in need of a peptide of the length of roughly 20 amino acids.
> Instead of
> having someone to synthesize it (who I will then have to pay), I
> thought of 
> expressing it into the periplasme of E.coli and purifying it via a
> His-tag. 
> However, small non-structured peptides of that length may be degraded
> by pro
> teases, so I would like to know if anyone has experience with such
> set-ups
> 
> 
> Thanks,
> 
> 
> Svend Kjaer,
> 
> IMSB,
> University of Aarhus,
> Denmark
> 



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