French press

[Rico Laage]

Dear colleagues

I´m starting to use a french press for breaking my bugs to purify a
recombinant protein, I have a problem what pressure I should use for E.
coli to completely crack the cells- I am operating aSLM Aminco with the
40K manual fill cell- thanx a lot for any suggestions-


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Rico Laage
Neurobiology
University of Heidelberg
Im Neuenheimer Feld 364
69120 Heidelberg
Germany
Voice  +49 (6221)54-4863      Fax    +49 (6221)54-4496
World-Wide-Web:
http://server.nbio.uni-heidelberg.de/Neurobiology.html
http://server.nbio.uni-heidelberg.de/Groups/WWW_Langosch/Rico_Laage.html

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