Expression of peptides in E.Coli

Svend Kjaer kjaer at biobase.dk
Wed Jun 10 04:22:12 EST 1998


Hi all,

I am in need of a peptide of the length of roughly 20 amino acids. Instead of
having someone to synthesize it (who I will then have to pay), I thought of 
expressing it into the periplasme of E.coli and purifying it via a His-tag. 
However, small non-structured peptides of that length may be degraded by pro
teases, so I would like to know if anyone has experience with such set-ups


Thanks,


Svend Kjaer,

IMSB,
University of Aarhus,
Denmark



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