Secreted proteins

[Rafael Maldonado]

J.S Good wrote:
> I want to screen the genome of a gram-negative pathogen for secreted
> proteins, perhaps by cloning genomic DNA fragments into an E. coli
> expression plasmid, placing the fragments upstream of a reporter gene.
> Have you had any experience with this, or can you suggest a suitable
> promoter/reporter/plasmid?

You need a phoA expression vector, available elsewhere. Make a medline
search with phoA and vector.

-Rafa

-- 
Rafael Maldonado
Division of Genetics
University of Alicante
rmaldonado at delete.ua.es 
(remove "delete." from the address to send e-mail)