Secreted proteins

Rafael Maldonado rmaldonado at ua.es
Tue Jun 9 14:00:37 EST 1998


J.S Good wrote:
> I want to screen the genome of a gram-negative pathogen for secreted
> proteins, perhaps by cloning genomic DNA fragments into an E. coli
> expression plasmid, placing the fragments upstream of a reporter gene.
> Have you had any experience with this, or can you suggest a suitable
> promoter/reporter/plasmid?

You need a phoA expression vector, available elsewhere. Make a medline
search with phoA and vector.

-Rafa

-- 
Rafael Maldonado
Division of Genetics
University of Alicante
rmaldonado at delete.ua.es 
(remove "delete." from the address to send e-mail)



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