competent cells again!!

[Rafael Maldonado]

John Augliera wrote:
> It makes no mention of adding a
> cryoprotectant such as glycerol.  Would anyone know if this is OK!! or
> would the addition
> 10% glycerol adversely affect the competency or viability of the cells.

Yes, glycerol will inhibit transformation; but you need of course a
cryoprotectant, and the best is 7% DMSO. There is an improved CaCl
method by Inoue in a Gene of the 80´s. If you want, I might check it for
you.
 
-Rafa

-- 
Rafael Maldonado
Division of Genetics
University of Alicante
rmaldonado at delete.ua.es 
(remove "delete." from the address to send e-mail)