Immunodetection of 40-50kDa proteins

rmeili at hotmail.com rmeili at hotmail.com
Wed Jun 10 21:03:42 EST 1998


Hi,

Protein A reagents which recognize only native IgG (rabbit, mo IgG 2a, mo
IgG 2b, mo IgG 3 ?) are often useful as well. Protein A can be bought
coupled to various molecules like alkaline phosphatase or horse raddish
peroxidase so it can directly replace your secondary antibody.

hope this helps





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