Maxipreps and columns (or not?)

Thibaud Le Mouël lemouel at antibes.antibes.inra.fr
Thu Jun 11 03:31:41 EST 1998


In article <6lm7t8$j5o at mserv1.dl.ac.uk>, wgschech at med.uni-tuebingen.de wrote:

>Hi all.
>Just run a quality control on a column maxiprep (NucleoBond from
>macherey-nagel). I noticed no difference in the look of the gel bands
>comparing the material I obtained after lysis and neutralization
>(KOAc step, i.e. that what I apply to the column) and the eluate from
>the column.
>So, I just wonder if all this fuss really is necessary (for doing
>transfections) or if simply a direct iPropOH precipitation would do
>it, too.
>Has anyone yet tried the transfection quality of not column or CsCl
>purified plasmids?
>
>The E. coli host I'm using is XL1-blue
>
Hi,

I usually use the QIAGEN Maxi Kit which is like Nucleobond and the DNA is
very good for transfection (better than 2 CsCl). In CsCl, you are not sure
to eliminate all the other things.

So go and good luck for transfection
Bye
Thib

****************************************
Th. Le Mouel  <lemouel at antibes.inra.fr.>
I.N.R.A.
123, bld F. Meilland
B.P. 2078
06606 ANTIBES CEDEX    FRANCE
****************************************

"Up the Irons..."





More information about the Methods mailing list