Solubilizing RNA in water

Keith Savin savink at vicnet.net.au
Fri Jun 12 23:14:37 EST 1998


Simon Mauch wrote:

> You could use (deionized) formamide instead of water. RNA (and other
> stuff) will solubilize very rapidly (up to 2-4 mg/ml) in formamide.
> I did some RT-PCR on RNA solubilized in formamide instead of water and
> had no problems with it. The direct comparison of water-RNA versus
> formamide-RNA showed the same RT-PCR results in my hands.
> 
> Simon

I think I could use that idea Simon, but how do you get rid of the
formamide? Doesn't it interfere with the RT ?

Keith



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