Solubilizing RNA in water
Simon.Mauch at uni-konstanz.de
Fri Jun 12 12:19:02 EST 1998
>I've been using a product called Tri-Reagent to isolate RNA from tobacco plants.
>I get large amounts of RNA but often the pellet is insoluble in water after the
>75% ethanol wash, even when I don't dry the pellet at all. I need to be able to
>go into an RT-PCR with my sample. Does anyone have a solution?
You could use (deionized) formamide instead of water. RNA (and other
stuff) will solubilize very rapidly (up to 2-4 mg/ml) in formamide.
I did some RT-PCR on RNA solubilized in formamide instead of water and
had no problems with it. The direct comparison of water-RNA versus
formamide-RNA showed the same RT-PCR results in my hands.
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