strange cloning results
william at neuro.usc.edu
Fri Jun 12 12:37:24 EST 1998
I've isolated several clones from a cDNA library with a labeled probe. When
I analyzed the clones, I found that many of them are in the reversed
orientation (the library was oligo-dT primed and uni-directionally cloned).
Is this just an artifact or can some gene have 2 ORFs in opposite directions?
Another strange clone that I isolated was that if I translate the aa in the
same frame as an already known homologous protein, I don't detect a stop codon -
it translates right through to the poly-A tail. I sequeced the clone in both
directions so I'm sure it's not a mistake in the sequencing. Can anyone offer
William Sun, Ph.D
Metabasis Therapeutics, Inc. Phone: (619) 622-3930
9390 Towne Centre Drive FAX: (619) 622-5545
San Diego, CA 92121 Email: william at alum.mit.edu
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