electroporation/use no steril glycerol

Christian Ebeling ebeling at mail.mpiem.gwdg.de
Fri Jun 12 11:35:00 EST 1998


hi bionetters,
anybody knows why BIORAD write in the manual "High Efficiency
Elektroporation of E.coli":...Do not autoclave or filter-sterilize the
glycerol solution...? I do everything to avoid contaminations, but in
this step of the manual I have to work not steril!?
ebel



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