Protein purification with protein-protein interaction

Thomas Döring doering at hpi.uni-hamburg.de
Thu Jun 11 07:10:28 EST 1998


Hi everybody,

I have a N-his tagged protein and want to use it to enrich nuclar proteins.
Therefor I wnat to immobilize the his tagged protein on Ni-NTA agarose,
let the nuclear proteins bind and at the end elute the bound proteins.
Does anyone have a suggestion to my idea? Can I do the whole prozedure in
a spin column. 

Thanks,

Thomas



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