Northern help

Gregg Silk greggsilk at aol.com
Sat Jun 13 16:46:38 EST 1998


Yes, that'll work if the levels of expression are high enough. You might not
detect low level transcripts this way. Slot or dot blot a couple ug of DNA band
(dilute, denature by boiling 10',  blot to membrane). Boil the total RNA in
tris pH 9.0 for 10' to degrade somewhat and give you mre free ends, end  label
with P32 using kinase, don't worry about cleanup, but TCA ppt and count.
Incorporation should be very high. Hyridize and wash at  high stringencies.
Gregg



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