rjv at riker.neoucom.edu
Sat Jun 13 17:26:21 EST 1998
I am interested in making a cell line from chicken corneal fibroblasts. I
realize that this is not a trivial undertaking, but i need to make a cell
line that retains much of its normal phenotype (good luck you say?). I
will characterize the lines with hybridization to an array of cDNAs that are
expressed in the differentiated cells.
I am looking for some input on what the best way immortalize these
cells might be. I am leaning toward using vMyc expressed from an avian
specific retrovirus perhaps with selectable marker too? I am a novice at
this and would like some advice on where to start reading.
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