ECL Reagents

Vittorio Verzillo verzillo at
Mon Jun 15 07:53:07 EST 1998

On 11 Jun 1998, Zac Pujic wrote:

I use these solutions
1)250 mM luminol (sigma a-8511) in DMSO; 0.44g in 10ml (keep light tight)
2)p-coumaric acid (sigma c-9008) 90 mM; 0.15g in 10ml DMSO
both in aliquots at -20.

at the moment mix together A + B:
A) 50 ul sol 1, 22 ul sol 2, 5 ml tris ph 8.5
B) 3ul h2o2 30% in 5 ml tris ph 8.5

good luck

> A few months ago, I saw several posts about making ECL reagents from
> commercially available sources of Luminol (3-aminophthalhydrazide) and
> 4-iodophenol. Some individuals gave the concentrations needed, and even
> the catalog numbers of some of these items. Does anyone have such info or
> is there a www site which contains it?
> Zac Pujic
> dnzpujic at
> Department of Dentistry
> The University of Queensland
> Queensland, Australia

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