Getting a second PCR product

Guenter Jost guenter.jost at io-warnemuende.de
Mon Jun 15 04:21:52 EST 1998


Using universal bacterial primers for 16S rDNA with a usual PCR protocoll we got the 
desired band at about 1500 bp but also a second one with about the double bp from 
E.c.
An additional run of a nested PCR for a DGGE product of about 500 bp with GC clamp
came up again with two bands, one was the right one and the second was again 
about double as long as the right one.
Any suggestions?
Thanks,
Guenter Jost
Guenter.Jost at io-warnemuende.de 




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