? Peptide solubilisation ?

[Steve Bryson]

Jongrak Kittiworakarn (g3937506 at STUDENT2.MAHIDOL.AC.TH) wrote:
: Dear All:

: I am going to purify a synthetic peptide by hplc, but I have a problem
: with its solubility.
: I have tried several solvent, i.e. CH3CN, water, 20-50% CH3CN in water,
: isopropanol, methanol, DMF, but the peptide just slightly soluble in
: those solvent.
: I would greatly appreciate, if you can give me any suggestion on the
: solubilisation of the peptide.

: Thanks in advance
: Jongrak



: --
: -----------------------------------Mr. Jongrak Kittiworakarn
:                                    g3937506 at student.mahidol.ac.th

:      Inst. of Science and Technology for Research and Development
:      Mahidol University (Salaya Campus)
:      Salaya, Nokornprathom, THAILAND.
:      Tel. 001-66-2-441-9003-7 ext.1277,1278,1279
:      Fax. 001-66-2-441-9906, 001-66-2-441-1013
: -----------------------------------------------------------------

Try adding a little concentrated NaOH or a strong buffer at high pH.
Both of these have worked for me in the past.