chrisb at hgu.mrc.ac.uk
Mon Jun 15 11:22:33 EST 1998
Robert DeLisio (QuintMark at earthlink.net) wrote:
: Decomtamination of pipets with UV is not effective.
: The UV light source must be perpendicular to the surface to be
: decomtaminated in order to inactivate amplifiable DNA. Pipets are 3-D and
: have rounded surfaces that are not exposed to sufficient UV to completely
: decontaminate. I've found that wiping down pipets ( or any other surface )
: with 10% bleach is the most effective way to get rid of contaminating DNA.
: Bleach should be used with caution, however. Should ANY bleach get into your
: reaction mixture it will kill it completely.
Another recently reported problem with UV decontamination for PCR is
Linquist, V., Stoddart, C. A. and McCune, J. M. (1998) `UV irradiation
of polystyrene pipets releases PCR inhibitors.' BioTechniques, 24, 50.
(The lack of any bad effects on polypropylene seems to be implied by
Chris Boyd | from, but not \ MRC Human Genetics Unit,
Christopher.Boyd at hgu.mrc.ac.uk | on behalf of / Western General Hospital,
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd \ Edinburgh, EH4 2XU, SCOTLAND
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