Superscript/RNAsin

Ben Wielockx ben at lmb1.rug.ac.be
Tue Jun 16 10:45:55 EST 1998


Lecocq Pierre wrote:
> 
> Dear Keith,
> 
> I don't know about use of RNAsin with superscriptII but if you take a look
> at Clontech Smart PCR kit, they say to avoid use of RNAsin. Also to other
> users that try to use clontech smart I have to say that in fact CAP finder
> primer efficiency is directly related to use of Gibco BRL RT (it's a RT
> that add GGGGGG ! I try other RT just like expand Boehringer and it does
> not work!).
> 
> Pierre Lecocq

I used the Gibco-BRL kit starting from mRNA in an elution buffer. But I
have no reverse transcription. Solution?
Is there a method to measure the exact concentration of the obtained
cDNA. (fenol extraction?)
Any advise is welcome
Thanks



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