Extra bands on SDS Page

Cres Bookstein cbookste at MEDICINE.BSD.UCHICAGO.EDU
Wed Jun 17 16:52:23 EST 1998


>Date: Wed, 17 Jun 1998 14:54:44 -0500
>From: Keld Sorensen <KSorense at notesgw.sial.com>
>Organization: Sigma
>Mime-Version: 1.0
>To: cbookste at medicine.bsd.uchicago.edu, J.Warawa at bristol.ac.uk
>Subject: Extra bands on SDS Page
>
>Cres is giving good advice !  - This is an experience many of us have
>had. At times, you have to start over with fresh reagents if they have
>become contaminated.
>
>Remember to WASH OUT the gel box (I prefer detergent such as
>SigmaClean rather than acid!)
>
>Sorry - I can not post to the newsgroup - our newsserver is acting up!
>
>Keld Sorensen, Manager Mol. Biol. R&D
>Sigma, PO BOX 14508, St. Louis MO 63178, USA
>web: http://www.sial.com
>------------------------------------------
>> Re: extra bands on SDS PAGE
>>
>> Cres Bookstein (cbookste at medicine.bsd.uchicago.edu)
>> 17 Jun 1998 12:35:49 -0700
>> From: cbookste at medicine.bsd.uchicago.edu (Cres Bookstein)
>> Subject: Re: extra bands on SDS PAGE
>>
>> Date: 17 Jun 1998 12:35:49 -0700
>>
>> To Jonathan Warawa: Frequently extra bands of approximate size you mention
>> are keratins which come from hands on the gel plates. Acid wash your gel
>> plates, clean out all the running and blotting setups, use gloves start to
>> finish, cover all after they have been cleaned and never let anyone touch
>> your gel stuff with bare hands.
>>
>> Crescence Bookstein, Ph.D.
>> Research Associate/Assistant Professor
>> Dept of Medicine-GI mc 6084
>> Univ. of Chicago
>> 5841 S. Maryland Ave.
>> Chicago, IL 60637
>> cbookste at medicine.bsd.uchicago.edu
>>
>> Phone: 773/702-2283 Fax: 773/702-2281
>>   ----------------------------------------------------------------------
>

Crescence Bookstein, Ph.D.
Research Associate/Assistant Professor
Dept of Medicine-GI  mc 6084
Univ. of Chicago
5841 S. Maryland Ave.
Chicago, IL 60637
cbookste at medicine.bsd.uchicago.edu

Phone: 773/702-2283             Fax: 773/702-2281





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