oligo-dT synthesised cDNA: 18s rRNA??

ÀÏ»¢Æ¨¹É han at biogate.com
Wed Jun 17 17:25:41 EST 1998


Dear folks,

I encountered a rather strange thing that I can't explain it. We synthesised
single stranded cDNA with oligo-dT primer from mRNA template pool.
Surprisingly we amplified a segment of 18s rRNA product. It is well known
18s rRNA havn't poly-A tail so how could we amplify a clear band?? Is it
possilbe there is genomic DNA contaminatin during RNA extraction? Is it the
only explanation? We use Trizol to extract total RNA from rat.

Thanks.

Huang





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