How to optimize EMSA?

Wei-Jen Chang wjc at acsu.buffalo.edu
Wed Jun 17 13:07:16 EST 1998


I am doing a Gel shift experiment.  Using a condition the book 
recommended seems not working.  I am thinking of changing the
DNA-Protein binding condition.  Does anyone know which condition
should be first considered (i.e. salt concentration or...)?

Thanks.

Wei-Jen Chang




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