TA cloning

Jun Zhang junz at sfu.ca
Wed Jun 17 13:10:21 EST 1998

Hi, Denni,

The kit recommands fresh PCR products. They even don't recommand gel
purified PCR product. I think the A tail is not quite stable. At least
lower the ligation efficiency. Good luck.


> > 
> Hi,
> I want to TA clone some PCR products, but these were kept at -20 C for a
> few weeks. Would this cause problems? Are there better ways (if any) of
> storing the PCR reactions? Also, what is people's experience with kits
> (TA, not blunt-end, and preferably cheap)? I am thinking about getting
> PGEM T vector from Promega, seem to be the cheapest in Europe.
> Regards,
> Denni
> Dept. Paediatric Gastroenterology 
> Bart's Hospital
> Dominion House 
> 59 St. Bartholomews Close
> London EC1A 7BE

More information about the Methods mailing list