TA cloning

Denni Schnapp dschnapp at molbiol.ox.ac.uk
Wed Jun 17 12:02:59 EST 1998


I want to TA clone some PCR products, but these were kept at -20 C for a
few weeks. Would this cause problems? Are there better ways (if any) of
storing the PCR reactions? Also, what is people's experience with kits
(TA, not blunt-end, and preferably cheap)? I am thinking about getting
PGEM T vector from Promega, seem to be the cheapest in Europe.



Dept. Paediatric Gastroenterology 
Bart's Hospital
Dominion House 
59 St. Bartholomews Close
London EC1A 7BE

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