TA cloning

Denni Schnapp dschnapp at molbiol.ox.ac.uk
Wed Jun 17 12:02:59 EST 1998


Hi,

I want to TA clone some PCR products, but these were kept at -20 C for a
few weeks. Would this cause problems? Are there better ways (if any) of
storing the PCR reactions? Also, what is people's experience with kits
(TA, not blunt-end, and preferably cheap)? I am thinking about getting
PGEM T vector from Promega, seem to be the cheapest in Europe.

Regards,

Denni



Dept. Paediatric Gastroenterology 
Bart's Hospital
Dominion House 
59 St. Bartholomews Close
London EC1A 7BE




More information about the Methods mailing list