How many T'?

Karin Zerulla zeru at zedat.fu-berlin.de
Wed Jun 17 09:21:32 EST 1998


yoramg gerchman schrieb:

> Dear netters, doe's any one know how many T' are hanging in the
> cohesive
> ends of T-vector?

T-vectors have a single 3'-T overhang at the insertion site, because
many thermostable polymerases add a single A to the 3'-ends of PCR
products.

Regards,
Karin




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