DNA extraction for PCR

Parent, Eric ParentE at dfo-mpo.gc.ca
Wed Jun 17 08:03:36 EST 1998


Hello all.

I have a problem amplifying DNA extracted from decapods. Do the use of
proteinase K somewhat inhibit polymerase even if a phenol extraction is
perform ? The DNA is precipipated with LiCl and resuspend in TE.  Is there a
potential problem there ?

any help would be greatly apreciated

Eric

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Eric Parent 
Invertebrates and experimental
biology division                              
Fisheries and Oceans Canada	
Maurice-Lamontagne Institute
Mont-joli, Qc
Canada

parente at dfo-mpo.gc.ca
http://www.qc.dfo-mpo.gc.ca/iml
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