epitope tag: two or one?

rmeili_nospamare at hotmail.com rmeili_nospamare at hotmail.com
Tue Jun 16 22:30:27 EST 1998


Hi all,

I am interested to hear what experience you have with a tandem repeat of an
epitope tag like myc, HA, FLAG etc. Since an IgG has two binding sites the
affinity should be much increased when compared to a single tag. 
Is there any literature you know about? Should there be a spacer between
the two tags? 

Thanks a lot

Ruedi





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