RACE-PCR

Desai Rooma bmrooma at WIZARD.WEIZMANN.AC.IL
Wed Jun 17 04:23:25 EST 1998


I'm trying to get the 5' and 3' sequence of a gene by RACE PCR. The
positive control with the two gene specific primers work, but the 5' and
3' RACE reactions don't. I see absolutely nothing there! Can any one
suggest what could be the problem. Also, is there any other way of getting
the complete gene sequence, besides RACE-PCR and cDNA library?

Any help will be greatly appretiated.

Thank you,
Rooma.

__________________________________________________________________________

Rooma Desai

Dept. of Neurobiology
Weizmann Inst. of Science
Rehovot, Israel

office phone: 934-2855




More information about the Methods mailing list