oligo-dT synthesised cDNA: 18s rRNA??

Gregg Silk greggsilk at aol.com
Wed Jun 17 17:32:15 EST 1998


Just idle speculation here (advice is free)...

1) rRNA genes come in multiple copies, so if there were any DNA contamination
it might show up there.
2) check the proximal region of 18S for a stretch of A's.
3) RNA has lots of secondary structure, so maybe it is self priming, esp if
there is some degradation.

Gregg 

Make mine a double!-anon







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