Tracey.Smith at UCHSC.EDU
Thu Jun 18 15:22:02 EST 1998
We have been trying to clone cDNA into either the T7 Select vector or
Stratagene's Lambda Zap vector. Regardless of the cDNA or the
adapters that we use, we consistantly only clone adapters and no
cDNA. We have used several types of column chromatography and NH4OAC
precipitations to try and clean the free adapters from our cDNA.
Even when we use our column chromatography and select fractions
that should contain only large DNA (>800bp), we will still only get
free adapters cloning. Has anyone else experienced this problem and
if so....how do you effectively get rid of all of those adapters?
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