Alamarblue proliferation assay ?
wgschech at med.uni-tuebingen.de
Thu Jun 18 11:31:28 EST 1998
Hi Jens Rainer.
I just can say that I tried Alamr Blue, too in order to measure the
influence of drugs on cell growth. With 3H-thymidin, there was a
clear signal, but Alamar Blue: No difference.
Maybe Alamar Blue is just good for making strong effects visible.
Did you make MTT or XTT assays? Did these work? with your cells?
> I have used 20 mcl alamarblue, 5x10**5 cels/ml, 200 mcl
> cellsusp.(human lymphocytes - PHA stimulated). The cells were
> preincubated for 48 hours and the alamarblue added hereafter and
> meassured at 570 and 600 nm after 2, 4, 6 and 24 hours on a
> microtiterplate reader.
> No significant difference was seen between the PHA stimulated and
> the basal cells. The assay is functioning with 3-h thymidine.
> I would be greatfull if you have any coments concerning using
> alamarblue .
> Yours Jens R. Hansen
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