TA cloning (the secret)
Dr. Duncan Clark
duncan at nospam.demon.co.uk
Thu Jun 18 03:36:29 EST 1998
In article <1998061722491600.SAA05060 at ladder03.news.aol.com>, Gregg Silk
<greggsilk at aol.com> writes
>If you don't use the PCR product
>*immediately,* you should do 1X chloroform extraction to kill the Taqpol!
Chloroform will not kill all the Taq pol. It is very hardy.
Just store your product at -20C. When you come to use it add a little
extra Taq pol and stick it on a PCR machine for 10mins at 70C to add the
A overhang again. Use immediately for TA cloning.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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