control in cRT-PCR
helene dauchel
helene.dauchel at univ-rouen.fr
Thu Jun 18 05:20:13 EST 1998
Hi,
I'm interesting in competitive RT-PCR. I want to use a RNAr probe as a
constitutive control. But I asked me if
-it is neccesary to make an RNA competitor for it? And then to do the
experiments so as my samples.
or -just a simply non quantitative RT-PCR then adjusted the
amounts of products by the levels of amplified DNA for ARNr
or others methods?
What is the best, practice and rigorous method for using a such
constitutive control?
Thanks for any help
helene
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