Source of vaccinia DNA Topoisomerase ?

bradley turner turner at sprcore.bidmc.harvard.edu
Thu Jun 18 11:50:41 EST 1998


Hi Martin,

I would also be interested in any sources of vaccinia topoisomerase
that you might obtain.  Could you email me with any results

Thanks, 
Brad Turner


**************************************************************
  		    Bradley Turner
                Beth Israel Deaconess
                    Medical Center

Harvard Medical School		617-667-1215 phone
Division of Gastroenterology	617-667-2767 fax
Room Dana 536			bsturner at mbcrr.harvard.edu
330 Brookline Avenue		bturner at bidmc.harvard.edu
Boston, MA 02215		turner at sprcore.bidmc.harvard.edu
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>From BIOSCI-REQUEST at net.bio.net Wed Jun  3 12:12:27 1998
Return-Path: <BIOSCI-REQUEST at net.bio.net>
To: methods at net.bio.net
From: Messerle at lmb.uni-muenchen.de (Martin Messerle)
Subject: Source of vaccinia DNA Topoisomerase ?
Date: 3 Jun 1998 03:10:43 -0700
Message-Id: <35752076.2266 at lmb.uni-muenchen.de>
Reply-To: Messerle at lmb.uni-muenchen.de
Nntp-Posting-Host: net.bio.net
Status: R

Hi,

I am looking for a source of vaccinia DNA topoisomerase. I know
Invitrogen is selling Topo Cloning Kits (they work very well and are
quite expensive :-((   ), however one is restricted to the provided
vectors with these kits. In the original paper (Shuman, J.Biol.Chem. 51,
32678-32648. 1994.) Shuman described a way how to use topoisomerase for
cloning in any vector; primers have to be modified in a certain way,
then, topoisomerase will bind to the PCR product.
However, this requires that somebody provides the enzyme.
Does anybody know a source for vaccinia DNA topoisomerase?

Thanks for your help,
Martin

---
Dr. Martin Messerle
Max von Pettenkofer-Institute
Genzentrum
Feodor-Lynen-Str. 25
81377 Munich, Germany



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