"Orange" pellet in plasmid mini-prep

Rafael Maldonado rmaldonado at jamon.ua.es
Fri Jun 19 10:46:44 EST 1998


Ray Boykiw wrote:

> One thing that appears slightly different is
> that when we pellet the bacteria from the broth culture, the pellet
> appears slightly orange compared to what we are used to.  

It sounds like you have got a contamination. Are you sure your strain is
still E. coli? Does it grow in McConkey medium, for instance?

-Rafa 

-- 
Rafael Maldonado
Division of Genetics
University of Alicante
rmaldonado at delete.ua.es 
(remove "delete." from the address to send e-mail)



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