"Orange" pellet in plasmid mini-prep

Rafael Maldonado rmaldonado at jamon.ua.es
Fri Jun 19 10:46:44 EST 1998

Ray Boykiw wrote:

> One thing that appears slightly different is
> that when we pellet the bacteria from the broth culture, the pellet
> appears slightly orange compared to what we are used to.  

It sounds like you have got a contamination. Are you sure your strain is
still E. coli? Does it grow in McConkey medium, for instance?


Rafael Maldonado
Division of Genetics
University of Alicante
rmaldonado at delete.ua.es 
(remove "delete." from the address to send e-mail)

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