epitope tag: two or one?
chrisb at hgu.mrc.ac.uk
Fri Jun 19 05:32:35 EST 1998
Darren A. Natale (dnatale at box-d.nih.gov.nospam) wrote:
: Guy Oshiro wrote:
: > It is commone to repeat the epitope tag. It is common to see 3xHA tags
: > and 9xMyc tags in the literture and in my clones. You get a much
: > better signal with the repeats than the single.
: Okay, so does anybody have vectors for these multiple tags? I (and
: likely others) would be interested in vectors that allow for N-terminal
: or C-terminal tags of hexahis, FLAG, c-myc, HA, and any other you might
It's far cheaper and more flexible to use PCR to add tags to order,
then clone into a single conventional vector.
Chris Boyd | from, but not \ MRC Human Genetics Unit,
Christopher.Boyd at hgu.mrc.ac.uk | on behalf of / Western General Hospital,
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd \ Edinburgh, EH4 2XU, SCOTLAND
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