vurk at hymail.hanyang.ac.kr
Fri Jun 19 02:38:22 EST 1998
1.Enzyme digestion, 2 Elution of digested DNA fragment , 3.
Dephosphorylation,and 4. Ligation
I did the whole step in 1->2->3->4..order.
However what if I do in 1->3->2->4.
What is more efficient ?
#1.Dephosphorylatiion before Elution or #2. Dephosphorylation
I did Elution by using BioRad Elution KIT. and I found that about
1/10(or less than 1/10) DNA was eluted.
The efficiency is too low. Is this low efficiency is in general?
In addition, I heard that the eluted DNA is hard to be ligated by
Do you have any idea about these?
Laboratory of Microbiology
Department of Biology
College of Natural Science
Haengdang-dong 17 Fax : 082-02-290-0952
Seoul, South Korea E-mail: vurk at hymail.hanyang.ac.kr
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